Journal: Translational Cancer Research

Article Title: MicroRNA-663a upregulation upon ARID1A depletion promotes the growth and migration of esophageal cancer cells by targeting FKBP8

doi: 10.21037/tcr-2025-1457

Figure Lengend Snippet: miR-663a is induced in esophageal cancer cells upon ARID1A depletion. (A) Bioinformatics analysis showed 14 differentially expressed miRs between esophageal cancer and adjacent normal tissues based on GEO datasets ( GSE157805 and GSE145198 ). (B) The protein expression of ARID1A in esophageal cancer cells transfected with shCtrl or ARID1A-targeting shRNAs was measured by Western blot analysis. GAPDH was used as the loading control. (C) Measurement of miR-663a levels in esophageal cancer cells transfected with indicated shRNAs. ***, P<0.001 vs . shCtrl. (D) The expression level of miR-663a in 50 pairs of esophageal cancer and adjacent normal tissues measured by quantitative real-time PCR analysis. (E) The correlation between the miR-663a and ARID1A mRNA expression levels in the 50 cases of esophageal cancer tissues. (F) Overall survival analysis in esophageal cancer patients according to the miR-663a expression level using the data from Kaplan-Meier plotter. Data in parentheses are 95% confidence interval. GEO, Gene Expression Omnibus; HR, hazard ratio; miR, microRNA; PCR, polymerase chain reaction; shCtrl, control shRNA; shRNA, short hairpin RNA.

Article Snippet: After blocking with 5% skimmed milk, the membranes were incubated with the primary antibodies against ARID1A (1:800; Cell Signaling Technology, Danvers, MA, USA), FKBP8 (1:800; Cell Signaling Technology), SIRT6 (1:800; Cell Signaling Technology), and GAPDH (1:5,000; Cell Signaling Technology) at 4 °C overnight.

Techniques: Expressing, Transfection, Western Blot, Control, Real-time Polymerase Chain Reaction, Gene Expression, Polymerase Chain Reaction, shRNA